1. Eur J Clin Microbiol Infect Dis. Jun;35(6) doi: /s Epub Mar Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack. The Solana Influenza A+B Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of influenza A and influenza B viral RNA in nasal .

Author: Akinogal Dir
Country: Benin
Language: English (Spanish)
Genre: Medical
Published (Last): 28 October 2007
Pages: 459
PDF File Size: 14.15 Mb
ePub File Size: 3.58 Mb
ISBN: 362-6-99297-188-8
Downloads: 65683
Price: Free* [*Free Regsitration Required]
Uploader: Mogami

Electron microscopy-counted stocks of influenza viruses A and B were used to develop a quantitative PCR format. The Annual Flu Shot: Data for the to influenza season indicates that Moreover, the assay can be used for direct virus quantification and can be easily implemented in routine viral diagnostic testing.

Daley P, et al. July Picard C, et al. Facts, Statistics, and You The flu, or influenza, is a contagious respiratory illness caused by viruses that infect the nose, throat, and sometimes the lungs. The diagnostic efficacy of the multiplex TaqMan-based PCR was determined by testing 98 clinical samples.

Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR

A quadrivalent vaccine protects against the same three viruses as the trivalent vaccine plus protection against an additional influenza B virus. It produces a mild respiratory illness in adults.

However, x study in adults with influenza A and influenza B found they both resulted in similar rates of illness and death. For example, although virus isolation via cell culturing can be a robust and sensitive method for the detection of limited numbers of viable virions, it is labor-intensive and depends on optimal sample transport for sensitive virus isolation.


Subtypes of the influenza A virus. Infrared saunas promise a number of health benefits, from weight loss and decreased stress levels to improved circulation and even better skin. Viewpoints Point-of-care diagnostics is a growing and dynamic industry helping influfnza improve clinical and economic outcomes globally. Prospective comparative study of innfluenza specimens and methods in diagnosing influenza in adults. Influenza virus infection is a highly contagious respiratory disease that can spread easily and that is responsible for considerable morbidity and mortality each year.

Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes.

INFLUENZA A & B – Science

Please review our privacy ijfluenza. You can also contract the virus by touching a surface that has the virus on it and then touching your nose or mouth. Antimicrobial Resistance Management C. These types of medications interfere with the release of virus from infected cells. People who contract either type can spread the virus to others from up to six feet away when they cough or sneeze.

Our rapid diagnostic products are changing how healthcare is delivered-extending diagnosis and health management from x lab, doctor’s office and hospital into people’s homes.

For example, the most common influenza A subtypes that circulate seasonally in humans are H1N1 and H3N2. Cost-effective and non-invasive detection of H. Therefore, rapid diagnosis is important not only for timely therapeutic intervention but also for the identification of a beginning influenza outbreak. Using conventional culturing, we could detect virus on day 7 only infpuenza one patient.

This type of analysis, however, is time-consuming, either because sub type-specific PCRs need to be performed or because the post-PCR analysis is complicated. But even when this happens, the vaccine still offers some protection. Abbott is a world leader in rapid diagnostics at the point of care, with a focus on cardio metabolic disease, infectious disease and influenzza.


Here, we describe a multiplex TaqMan-based real-time PCR assay for the rapid and simultaneous detection of influenza viruses influenza influenaz A, influenza virus B, or both in clinical specimens. While most confirmed infections during flu season will be influenza A, the occurrence of influenza B infections can increase late in the flu season.

Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR

The fold serially diluted concentrations of the two strains were then amplified using the multiplex TaqMan assay. National Center for Biotechnology InformationU. Each territory in Africa is assigned to a specific Alere Business Manager who will have the responsibility to support you for all the essential commercial activities.

Rapid diagnostic tests with increased sensitivity are essential for the reliable detection of influenza A and B and enable immediate, effective treatment decisions.

These subtypes are based off of the combination of two proteins on the viral surface: It’s Not Just for Kids Anymore! They have proven to be very sensitive and specific but, unfortunately, are often difficult to implement in a routine diagnostic setting and still require influenxa sample handling and post-PCR analysis 135.